Engineered yeast strains with signal sequence-modified glucoamylase polypeptides and enhanced ethanol production
US11421212B2 · kind B2 · utility
2Cited by
23References
12Claims
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Key dates
| Filing date | Aug 4, 2017 |
| Grant date | Aug 23, 2022 |
| Priority date | — |
| Expiry date | Aug 4, 2037 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.