Ribosomal ribonucleic acid hybridization for organism identification

US11441196B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• The General Hospital Corporation · US

Inventors

• Roby Bhattacharyya · Cambridge, US
• Deborah Hung · Cambridge, US
• Jonathan Livny · Cambridge, US

Key dates

• Filing date: Mar 14, 2014
• Grant date: Sep 13, 2022
• Expiry date: Oct 31, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/158
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure relates to method of distinguishing between two or more species of one or more organisms in a sample, by contacting a biological sample comprising ribosomal ribonucleic acid (rRNA) with a set of antisense probes, wherein the set of probes contains at least one detectable probe that is specific for a target rRNA sequence of each species to be tested, and wherein the individual probes specific for each species comprises less than about 85% sequence identity; and, detecting hybridization between one or more of the probes and the rRNA, thereby distinguishing between two or more species in a sample.