Patent · US Active

Import of unnatural or modified nucleoside triphosphates into cells via nucleic acid triphosphate transporters

US11466279B2 · kind B2 · utility

2Cited by
198References
18Claims
0Family size

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Key dates

Filing dateOct 2, 2019
Grant dateOct 11, 2022
Priority date—
Expiry dateDec 23, 2039

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12P19/34
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A recombinantly expressed nucleotide triphosphate transporter efficiently imports the triphosphates of unnatural nucleotides into cells, and the endogenous cellular machinery incorporates those nucleotides into cellular nucleic acids. UBPs can therefore form within the cell's nucleic acids. Moreover, neither the presence of the unnatural triphosphates nor the replication of the UBP represents a significant growth burden. The UBP is not efficiently excised by nucleic acid repair pathways, and therefore can be retained as long as the unnatural triphosphates are available in the growth medium. Thus, the resulting cell is the first organism to stably propagate an expanded genetic alphabet.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.