Monitoring state deviations in bioreactors

US11473042B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Detlef Eisenkraetzer · Iffeldorf, DE
• Christian Klinger · Penzberg, DE
• Katrin Greppmair · Penzberg, DE
• Claudia Schmidberger · Altheim ob Weihung, DE

Key dates

• Filing date: Oct 28, 2016
• Grant date: Oct 18, 2022
• Expiry date: Mar 19, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/404
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a system (100) for monitoring deviations of a state of a cell culture in a bioreactor (104, 106) from a reference state of a cell culture in a reference bioreactor (102). The bioreactor comprises the same medium (M1) as the reference bioreactor. The system comprises: •—a storage medium (114) comprising: •a PACO-reference profile (116) indicative of a deviation of a CO2 off gas rate (ACOR-M-ti) measured in the reference bioreactor from a predicted CO2 off gas rate (ACOR-EXP-ti) of the reference bioreactor; •a data object comprising a medium-specific relation (136) between the pH value of the medium (M1) and a respective fraction of CO2 gas in a gas volume when said medium is in pH-CO2 equilibrium state with said gas volume and lacks the cell culture; •—an interface (128) for receiving (212) a current CO2 off gas rate (ACOBi-M-ti, ACOB2-M-t i) and a current pH value (pHBi-ti) of the medium of the bioreactor (104, 106); •—a comparison unit (130) configured for computing (214, 216): •a PACO value (PACOB1-tir PACOBi-ti) the PACO-value being indicative of a deviation of a CO2 off gas rate (ACOBi-M-ti, ACOB2-M-ti) measured in the bioreactor from a predicted CO2 of…