Protein purification and virus inactivation with alkyl glycosides

US11479578B2 · kind B2 · utility

Assignee

• CSL BEHRING LENGNAU AG · CH

Inventors

• Tobias BRANDT · Marburg, DE
• Hubert Metzner · Marburg, DE
• Carsten Horn · Ettenheim, DE
• Thomas Nowak · Staufenberg, DE

Key dates

• Filing date: Dec 19, 2018
• Grant date: Oct 25, 2022
• Expiry date: Nov 6, 2039

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61L2202/21
• WIPO field: Medical technology
• WIPO sector: Instruments

Abstract

A process for purifying a recombinant protein comprising the steps of: i) providing a solution comprising the recombinant protein; ii) adding an alkyl glycoside to the solution; and iii) purifying the recombinant protein. The addition of the alkyl glycoside provides improved clearance of process-related impurities. The purified recombinant protein of the invention has low levels of host cell DNA, host cell protein and viral contamination.