Antigenic tripeptides derived from Mycobacterium avium subsp. paratuberculosis s-type strains, derivatives and uses thereof

US11498942B2 · kind B2 · utility

Assignees

• INSTITUT PASTEUR · FR
• INSTITUT NATIONAL DE LA RECHERCHE AGRONOMQIUE · FR
• The United States of America, as represented by the Secretary of Agriculture · US

Inventors

• John P. Bannantine · Ames, US
• Gilles Etienne · Toulouse, FR
• Sylvie Bay · Paris, FR
• Franck Biet · Saint-Cyr-sur-Loire, FR

Key dates

• Filing date: Dec 20, 2017
• Grant date: Nov 15, 2022
• Expiry date: May 15, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/26
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention is directed to an isolated synthetic tripeptide of formula H-D-Phe-N-Methyl-L-Val-L-Ala-OMe (SEQ ID NO:1), or a derivative thereof, and to the corresponding lipotripeptides, which are specific to Mycobacterium avium subsp. paratuberculosis (Map) S-type strain, as well as derivatives and conjugates thereof. The invention also concerns the use of these antigens in different methods and tests for detecting Map infection, especially by detecting humoral response and cell mediated response of infected animals. The invention is also directed to a genetic signature of Map and a mass spectrometry and NMR spectroscopy signature of Map presence or infection.