Oligonucleotide-coupled antibodies for single cell or single complex protein measurements
US11549135B2 · kind B2 · utility
Assignees
Inventor
Key dates
| Filing date | Sep 13, 2019 |
| Grant date | Jan 10, 2023 |
| Priority date | — |
| Expiry date | Jun 11, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B70/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are compositions and methods for simultaneously measuring target oligonucleotides and protein in single cells. Compositions comprise an antibody-tagged oligonucleotide, including an origin specific barcode handle sequence, a first primer handle sequence, a second primer handle sequence, and a target binding region. The composition may also include an adapter sequence, a unique molecular identifier (UMI), and a poly-A sequence. Methods for simultaneously measuring target oligonucleotides and protein in single cells generally involve delivering a mixture of the composition to a population of cells and encapsulating individual cells in an individual discrete volume comprising PCR primers on a bead. The individual discrete volume may be suspended in a reverse transcription mixture and the nucleotide sequence of the origin specific barcode handle sequence may be detected, thereby assigning the target oligonucleotide and protein of interest to a specific individual discrete volume, while maintaining information about sample origin of the target oligonucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.