Method for tissue sample fixation
US11624684B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 22, 2018 |
| Grant date | Apr 11, 2023 |
| Priority date | — |
| Expiry date | Jul 16, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N1/31
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0° C. to at least 15° C. The second temperature typically is from greater than about 22° C. to about 55° C., and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.