Functional genomics using CRISPR-Cas systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof

US11685917B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US
• Children's Medical Center Corporation · US

Inventors

• Daniel E. Bauer · Cambridge, US
• Stuart H. Orkin · Brookline, US
• Neville Espi Sanjana · New York, US
• Ophir Shalem · Albany, US
• Jason Boke Wright · Concord, US
• Feng Zhang · Cambridge, US

Key dates

• Filing date: Nov 8, 2017
• Grant date: Jun 27, 2023
• Expiry date: Nov 8, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1082
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.