Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

US11691817B2 · kind B2 · utility

Assignee

• CARGILL, INCORPORATED · US

Inventors

• Catherine Asleson Dundon · Englewood, US
• Pirkko Suominen · Helsinki, FI
• Aristos Aristidou · Highlands Ranch, US
• Brian J. Rush · Minneapolis, US
• Kari Koivuranta · Helsinki, FI
• Benjamin Matthew Hause · Currie, US
• Thomas W. McMullin · Manitowoc, US
• Kevin Roberg-Perez · Minneapolis, US

Key dates

• Filing date: Jan 21, 2021
• Grant date: Jul 4, 2023
• Expiry date: Jan 21, 2041

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T137/85986
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyveromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.