Single cell cellular component enrichment from barcoded sequencing libraries
US11702649B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 23, 2018 |
| Grant date | Jul 18, 2023 |
| Priority date | — |
| Expiry date | May 15, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/02027
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the analysis of complex single cell sequencing libraries. Disclosed are methods for enrichment of library members based on the presence of cell-of origin barcodes to identify and concentrate DNA that is relevant to interesting cells or components that would be expensive or difficult to study otherwise. Also, disclosed are methods of capturing cDNA library molecules by use of CRISPR systems, hybridization or PCR. The present invention allows for identifying the properties of rare cells in single cell RNA-seq data and accurately profile them through clustering approaches. Further information on transcript abundances from subpopulations of single cells can be analyzed at a lower sequencing effort. The methods also allow for linking TCR alpha and beta chains at the single cell level.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.