Multiplexed genetic reporter assays and compositions

US11767534B2 · kind B2 · utility

Assignee

• The Broad Institute, Inc. · US

Inventors

• Tarjei Sigurd Mikkelsen · Dublin, US
• Andreas Gnirke · Wellesley, US
• Alexandre Melnikov · Bellingham, US
• Eric S. Lander · Cambridge, US
• Li Wang · Brookline, US
• Xiaolan Zhang · Chappaqua, US

Key dates

• Filing date: May 4, 2012
• Grant date: Sep 26, 2023
• Expiry date: May 4, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1086
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides methods for determining the activity of a plurality of nucleic acid regulatory elements. These methods may facilitate, e.g., the systematic reverse engineering, and optimization of mammalian cis-regulatory elements at high resolution and at a large scale. The method may include integration of multiplexed DNA synthesis and sequencing technologies to generate and quantify the transcriptional regulatory activity of e.g., thousands of arbitrary DNA sequences in parallel in cell-based as says (e.g., mammalian cell based assays).