Proteomic analysis with nucleic acid identifiers

US11873483B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• ÉCOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS (ESPCI PARIS TECH) · FR

Inventors

• Robert Nicol · Le Kremlin-Bicêtre, FR
• Andrew David Griffiths · Cambridge, GB
• Baptiste Saudemont · Fontenay-aux-Roses, FR
• Timothy V. Kirk · Paris, FR

Key dates

• Filing date: Mar 11, 2016
• Grant date: Jan 16, 2024
• Expiry date: Mar 16, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The disclosure provides methods and compositions useful for labeling of target molecules with origin-specific nucleic acid identifiers (for example, barcodes), which can be used subsequently to identify, quantify, or otherwise characterize a feature or activity of target molecules originating from a particular discreet volume. Such target molecules can include polypeptides expressed by cells, in which nucleic acid molecules encoding the polypeptides are labeled with the same, or matched, origin-specific nucleic acid identifiers.