High-throughput assembly of genetic elements
US11898141B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 27, 2015 |
| Grant date | Feb 13, 2024 |
| Priority date | — |
| Expiry date | May 27, 2035 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00722
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method of generating a set of sequence-verified nucleic acid elements for the combinatorial construction of genetic elements. The method includes: providing a plurality of nucleic acid parts; assembling nucleic acid parts to form a one or more nucleic acid elements, wherein the nucleic acid elements include at least two sequences selected from the plurality of parts; and determining the sequence of the nucleic acid elements. Further disclosed is a pool of higher-order nucleic acid constructs or amplification products thereof, comprising one or more nucleic acid elements as well as kits including a pool of sequence-verified nucleic acid elements of claims and/or a pool of higher-order nucleic acid constructs; and a plurality of primers for retrieving one or more sequence-verified nucleic acid elements and/or higher-order nucleic acid constructs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.