Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence

US11912985B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• President and Fellows of Harvard College · US

Inventors

• David R. Liu · Lexington, US
• Andrew Vito Anzalone · Cambridge, US
• Jonathan Ma Levy · Boston, US
• Xin Gao · Chengdu, CN
• Christopher J. Podracky · Cambridge, US

Key dates

• Filing date: Nov 7, 2022
• Grant date: Feb 27, 2024
• Expiry date: Nov 7, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/3519
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides systems, compositions, and methods for simultaneously editing both strands of a double-stranded DNA sequence at a target site to be edited. Further provided herein are pharmaceutical compositions, polynucleotides, vectors, cells, and kits for simultaneously editing both strands of a double-stranded DNA sequence.