Evolution of cytidine deaminases
US11913044B2 · kind B2 · utility
1Cited by
44References
21Claims
0Family size
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Key dates
| Filing date | Jun 14, 2019 |
| Grant date | Feb 27, 2024 |
| Priority date | — |
| Expiry date | Jun 14, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y305/04005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Some aspects of this disclosure relate to strategies, systems, methods, compositions, and kits that are useful for production (e.g., evolution) of cytidine deaminase protein variants that are characterized by increased soluble expression and/or stability relative to the wild-type cytidine deaminase protein from which they are evolved. In some embodiments, evolved cytidine deaminase variants described by the disclosure are useful for incorporation into targeted nucleic acid editing proteins, for example in fusion proteins with a Cas9 domain or variant thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.