Patent · US Active

B-cell cultivation method

US11952586B2 · kind B2 · utility

0Cited by

2References

20Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Sonja Offner · Penzberg, DE
Friederike Jung · Penzberg, DE

Key dates

Filing date	May 29, 2020
Grant date	Apr 9, 2024
Priority date	—
Expiry date	Apr 1, 2041

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2529/00
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Herein is reported a method for the co-cultivation of single deposited B-cells, which can be of any source, with EL-4 B5 feeder cells in a suitable co-cultivation medium. In the herein reported methods the EL-4 B5 cells have been irradiated with a dose of less than 40 Gy, preferably 9.5 Gy or less. Thereby the EL-4 B5 cells have a higher viability and maintain the ability to divide in cultivation at doses less than 6 Gy.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.