Method for determining the in vivo interaction mode

US11958912B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Joerg Moelleken · Penzberg, DE
• Michael Molhoj · Munich, DE
• Christian Gassner · Penzberg, DE
• Manuel Endesfelder · Weßling, DE
• Joerg Thomas Regula · Munich, DE

Key dates

• Filing date: Oct 2, 2020
• Grant date: Apr 16, 2024
• Expiry date: Aug 10, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/96466
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Herein is reported a method for determining the binding interaction with a multimeric antigen of an antibody of the human IgG1 subclass that has at least two binding sites specifically binding to the antigen comprising the steps of 1) determining the binding affinity of the antibody for the multimeric antigen, and 2) incubating a mixture comprising the antibody and a polypeptide that is derived from lysine-gingipain of Porphyromonas gingivalis under conditions and for a time sufficient to cleave the antibody into Fabs and Fc-region, and determining the binding affinity of the Fabs of the antibody for the multimeric, whereby the binding affinity of the antibody to the multimeric antigen to be affinity-driven if the binding affinity determined in both steps are comparable and to be avidity-driven if the binding affinity determined in both steps are different.