Compositions and methods for regulating proteins and nucleic acids activities

US11965159B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• The Brigham and Women's Hospital, Inc. · US
• The Regents of the University of California · US

Inventors

• Amit Choudhary · Boston, US
• Kurt Cox · Cambridge, US
• Hari K. K. Subramanian · San Diego, US
• Elisa Franco · Oakland, US

Key dates

• Filing date: Jan 29, 2020
• Grant date: Apr 23, 2024
• Expiry date: Jun 20, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Compositions and methods for conditionally regulating activities of CRISPR-Cas systems. In some embodiments, the methods comprise providing an inactive guide RNA comprising a regulatory domain bound by a lock nucleic acid different from the guide RNA; and displacing the lock nuclei acid from the regulatory domain by a trigger nucleic acid, thereby activating the guide RNA, wherein the activated guide RNA forms a complex with a Cas enzyme.