Agarase mutant with improved thermal stability and application thereof
US11999981B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 19, 2023 |
| Grant date | Jun 4, 2024 |
| Priority date | — |
| Expiry date | Oct 19, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01081
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.