DNA polymerases for efficient and effective incorporation of methylated-dNTPS
US12043851B2 · kind B2 · utility
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10References
23Claims
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Assignee
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Key dates
| Filing date | Mar 21, 2019 |
| Grant date | Jul 23, 2024 |
| Priority date | — |
| Expiry date | Aug 29, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are DNA polymerases having improved ability to incorporate methylated-dNTPs, relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases. Further disclosed are kits and reaction mixtures comprising the improved DNA polymerases as well as methods of primer extension using the improved DNA polymerases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.