Attenuation of human respiratory syncytial virus by genome scale codon-pair deoptimization

US12054749B2 · kind B2 · utility

Assignees

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US
• The Research Foundation for The State University of New York · US

Inventors

• Peter L. Collins · Kensington, US
• Cyril Le Nouen · Bethesda, US
• Linda G. Brock · Wyckoff, US
• Ursula Buchholz · Silver Spring, US
• Joshua Marc DiNapoli · Lexington, US
• Steffen Mueller · Pforzheim, DE
• Eckard Wimmer · Port Jefferson, US

Key dates

• Filing date: Jun 2, 2022
• Grant date: Aug 6, 2024
• Expiry date: Oct 14, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/18562
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Described herein are RSV polynucleotide sequences that make use of multiple codons that are containing silent nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce a numerous synonymous codons into the genome. Due to the large number of defects involved, the attenuated viruses disclosed herein provide a means of producing attenuated, live vaccines against RSV.