Mass spectrometry-based method for simultaneous qualitative and quantitative protein citrullination analysis of complex biological samples
US12111322B2 · kind B2 · utility
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Key dates
| Filing date | Dec 14, 2020 |
| Grant date | Oct 8, 2024 |
| Priority date | — |
| Expiry date | Aug 11, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/15
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention discloses mass spectrometry (MS)-based methods and tagging reagents for qualitative and quantitative analysis of post-translational modified (PTM) proteins in biological and clinical samples. The present invention utilizes thiol-containing tagging reagents which are able to bind to or derivatize biomolecules, preferably post-translational modified (PTM) polypeptides such as citrullinated and homocitrullinated polypeptides. The tagging reagents are preferably biotin-derived tags able to react with a side chain of the modified polypeptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.