Microscope and method of microscopy for providing illumination light and generating an illumination pattern

US12146832B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Daniel Schwedt · Weimar, DE
• Tiemo Anhut · Jena, DE

Key dates

• Filing date: Oct 23, 2019
• Grant date: Nov 19, 2024
• Expiry date: Nov 28, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/367
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A microscope and method of microscopy having a light source for providing illumination light, a controllable manipulation device for generating in a variable manner an illumination pattern of the illumination light to be selected, an illumination beam path with a microscope lens for guiding the illumination pattern to a sample to be examined, a detector having a plurality of pixels for examining the fluorescent light emitted by the sample, a detection beam path for guiding the fluorescent light emitted by the sample to the detector, a main beam splitter for splitting illumination light and fluorescent light, a control and evaluation unit for controlling the manipulation device and for evaluating the data measured by the detector. The manipulation device is arranged in the illumination beam path upstream from the main beam splitter such that the pixel of the detector can be individually activated using the control and evaluation unit and in read out patterns to be selected.