Compositions, methods, kits and devices for molecular analysis
US12153014B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 10, 2023 |
| Grant date | Nov 26, 2024 |
| Priority date | — |
| Expiry date | Feb 10, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC08L2205/025
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.