Sample preparation, processing and analysis systems

US12168798B2 · kind B2 · utility

Assignee

• INTEGENX INC. · US

Inventors

• David Eberhart · Santa Clara, US
• Yuan Li · Dublin, US
• James Ogg · Sunnyvale, US
• Ezra Van Gelder · Palo Alto, US
• Stephen J. Williams · Scottsdale, US
• Timothy Woudenberg · Bethel, US
• Dean S. Burgi · Menlo Park, US
• William D. Nielsen · San Jose, US

Key dates

• Filing date: Dec 11, 2020
• Grant date: Dec 17, 2024
• Expiry date: Aug 11, 2043

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2035/00158
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.