System and method for sample preservation
US12181387B2 · kind B2 · utility
0Cited by
3References
5Claims
0Family size
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Key dates
| Filing date | Mar 13, 2023 |
| Grant date | Dec 31, 2024 |
| Priority date | — |
| Expiry date | Mar 13, 2043 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2291/02809
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is disclosed that permits calculation of reagent concentrations (in SI units) over time and space within a tissue sample as the sample is immersed in the reagent and the reagent diffuses into the tissue sample. The disclosed method has yielded the surprising result that once a formaldehyde concentration at all points within a tissue sample exceeds about 90 mM during a cold step of a cold+hot fixation protocol, the hot step of the fixation protocol can be commenced to provide reliable detection of molecular targets and preservation of tissue morphology in downstream analyses.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.