Method for obtaining regenerated seedlings of Brassica campestris L.ssp.chinensis from embryonic tip tissue
US12201076B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 30, 2024 |
| Grant date | Jan 21, 2025 |
| Priority date | — |
| Expiry date | Apr 30, 2044 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P60/40
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
A method for obtaining regenerated seedlings of Brassica campestris L. ssp. chinensis from embryonic tip tissues, including the following steps. A seed is inoculated to a germination medium for dark culture for 60 h. The testa, root tip, two cotyledons and middle growing point of the resultant germinated seed are removed, and an embryonic tip with a length of 3-5 mm is retained as an explant. The explant is sequentially subjected to low-temperature pre-culture in a pre-culture medium for 36 h, room-temperature shaking culture in a liquid bud induction culture medium for 10 min, and bud induction culture in a bud induction culture medium for 20 d. A regenerated plant with 5-6 leaves is transferred to a rooting medium for rooting culture for about 2 weeks, and a well-rooted plant is collected, and subjected to hardening and transplantation into a filed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.