Method of preparing silanols with selective cytochrome P450 variants and related compounds and compositions

US12227529B2 · kind B2 · utility

Assignees

• Dow Silicones Corporation · US
• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• John Roberts · Oklahoma City, US
• Dimitris Katsoulis · Midland, US
• Susanne Baehr · Pasadena, US
• Sabine Brinkmann-Chen · Glendale, US
• S. B. Jennifer Kan · Pasadena, US
• Frances H. Arnold · Pasadena, US

Key dates

• Filing date: Feb 19, 2021
• Grant date: Feb 18, 2025
• Expiry date: Oct 15, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y114/14001
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

This disclosure provides a method of preparing a silanol-functional organosilicon compound with a cytochrome P450 variant that facilitates the oxidization of a silyl hydride group to a silanol group in the presence of oxygen. The method includes combining the cytochrome P450 variant and an organosilicon compound having at least one silicon-bonded hydrogen atom to give a reaction mixture and exposing the reaction mixture to oxygen to oxidize the organosilicon compound, thereby preparing the silanol-functional organosilicon compound. Cytochrome P450 variants suitable for use in the method are also disclosed, along with methods for engineering and optimizing the same. Nucleic acids encoding the cytochrome P450 variants and compositions, expression vectors, and host cells including the same are also disclosed.