Redox-labile fluorescent probes and their surface immobilization methods for the detection of metabolites
US12228568B2 · kind B2 · utility
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23Claims
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Key dates
| Filing date | Jul 8, 2019 |
| Grant date | Feb 18, 2025 |
| Priority date | — |
| Expiry date | May 17, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC09K2211/1018
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein is an analytical method for profiling lactate (or other analyte) production in single cells, via the use of coupled enzyme reactions on surface-grafted resazurin/resorufin molecules is provided. Additionally surface-bound resazurin/resorufin that retains redox indicator activity as well as microfluidic devices comprising such surface-bound resazurin/resorufin indicators are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.