Automated system for high-throughput microinjection of adherent cells
US12233406B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 4, 2021 |
| Grant date | Feb 25, 2025 |
| Priority date | — |
| Expiry date | Dec 28, 2043 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6439
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Two or more micropipettes are used to increase a microinjection throughput in an automated system for microinjecting adherent cells on a Petri dish. In the system, a motorized stage carrying the Petri dish sequentially visits the cells according to an optimized injection sequence. The sequence is selected by minimizing a total distance traveled by the motorized stage such that each cell is visited once by one of the micropipettes. Using multiple micropipettes advantageously reduces the minimized total distance over using a single micropipette to thereby increase the throughput. The optimized injection sequence is obtained by solving an equality-generalized traveling salesman problem. Each micropipette is mounted on a motorized micromanipulator. The motorized stage and motorized micromanipulators operate coordinately that each micromanipulator goes down or up during movement of the motorized stage to compensate for unevenness between a focus plane and a moving trajectory of the motorized stage.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.