Method for monitoring an immersion fluid in a microscope

US12235432B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Thomas Ohrt · Golmsdorf, DE
• Daniel Haase · Zöllnitz, DE

Key dates

• Filing date: Aug 3, 2021
• Grant date: Feb 25, 2025
• Expiry date: Feb 7, 2043

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/10152
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provision is made for a method of monitoring an immersion fluid in a microscope having a lens which images a sample located on a sample carrier. In a step a), a camera is positioned which has an image field which is oriented in such a way that it captures the sample carrier and a space between the sample carrier and the lens and adjoining the sample carrier towards the lens, which space is used to receive the immersion fluid. In a step b), the immersion fluid is applied into the space between the sample carrier and the lens. In step c), an image with the immersion fluid being in the space between the sample carrier and the lens is recorded, and in a step d), the position, the area and/or the contour of the immersion fluid on the sample carrier from the image recorded in step d) are/is determined.