Method for producing sulfated polysaccharide and method for producing PAPS
US12264350B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 2, 2021 |
| Grant date | Apr 1, 2025 |
| Priority date | — |
| Expiry date | May 16, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/15
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method for producing sulfated polysaccharides by reacting a PAPS production/regeneration system utilizing the metabolic activity of a microorganism or a treated matter thereof with a microorganism expressing a sulfation enzyme or a treated matter or extract thereof upon mixing of inexpensive raw materials such as magnesium sulfate. The invention also provides a method for producing PAPS from inexpensive raw materials. The methods involve preparing a transformant (a) of a bacterium of the genus Corynebacterium, which contains a gene encoding an ATP sulfurylase and a gene encoding an APS kinase, which are expressible, and in which a cell plasma membrane of the transformant (a) is substance-permeable, or a treated matter of the transformant (a), and conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.