Method and apparatus for analysis of a sample by light sheet microscopy

US12313828B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Manuel Amthor · Jena, DE
• Daniel Haase · Zöllnitz, DE
• Thomas Kalkbrenner · Jena, DE
• Jörg Siebenmorgen · Jena, DE

Key dates

• Filing date: Feb 25, 2022
• Grant date: May 27, 2025
• Expiry date: Nov 24, 2043

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/30168
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method for light sheet microscope examination of a specimen, the specimen being illuminated with a light sheet by an illumination objective, and a light sheet microscope for performing the method. Light emitted by the specimen is imaged onto an area-type detector by a detection objective. An optical axis of the detection objective encloses with an optical axis of the illumination objective an angle that differs from 0° and from 180° and intersects the light sheet in a light sheet plane. The area-type detector records an image. A neural network analyzes the image to determine whether the light sheet plane is located in a focal plane of the detection objective, and/or in which direction along the optical axis the light sheet plane is located from the focal plane, and/or at what distance, measured along the optical axis of the detection objective, the light sheet plane is from the focal plane.