Patent · US Active

Optimized method for antibody capturing by mixed mode chromatography

US12331075B2 · kind B2 · utility

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11Claims

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Assignee

Hoffmann-La Roche Inc. · US

Inventors

Roberto Falkenstein · Munich, DE
Thorsten Lemm · Kochel am See, DE
Markus Strasser · Penzberg, DE
Hidenari Yamada · Tokyo, JP

Key dates

Filing date	Jan 31, 2020
Grant date	Jun 17, 2025
Priority date	—
Expiry date	May 20, 2043

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K16/2863
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Herein is reported a method for the purification of an antibody directly captured from clarified cell culture supernatants using Streamline CST and/or Capto MMC, wherein especially product related (aggregates and fragments) and process related impurities (host cell protein, media components) could efficiently be removed, resulting in a preparation with a purity comparable to classical protein A affinity chromatography.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.