Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)

US12359218B2 · kind B2 · utility

Assignees

• President and Fellows of Harvard College · US
• The Broad Institute, Inc. · US

Inventors

• David R. Liu · Lexington, US
• Benjamin Thuronyi · Cambridge, US
• Christopher Gerard Wilson · Berkeley, US

Key dates

• Filing date: Mar 3, 2023
• Grant date: Jul 15, 2025
• Expiry date: Mar 3, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y305/04005
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The instant specification provides for evolved base editors which overcome deficiencies of those in art (including increased efficiency and/or decreased requirement for specific sequence-context at an editing site) and which are obtained a result of a phage-assisted continuous evolution (PACE) system. In particular, the instant specification provides for evolved cytidine base editors (e.g., based on APOBEC1, CDA, or AID cytidine deaminase domains) which overcome deficiencies of those in art (including increased efficiency and/or decreased requirement for specific sequence-context at an editing site) and which are obtained a result of a phage-assisted continuous evolution (PACE) system.