Patent · US Active

Construction method and application of microRNA electrochemical biosensor

US12360075B1 · kind B1 · utility

0Cited by

0References

6Claims

0Family size

Assignee

Xuzhou Central Hospital · CN

Inventors

Yujuan Qi · Xuzhou, CN
Zhao Liu · Beijing, CN
Sen Zheng · Xuzhou, CN
Zhenbei Li · Xuzhou, CN
Juan Gu · Lo Wu, CN
Fangfang Hu · Xuzhou, CN
Yijuan Cao · Xuzhou, CN
Qingqing Sun · Shanghai, CN
Xiaoyan Liu · Beijing, CN
Nan Jiang · Tangxia, CN

Key dates

Filing date	Nov 8, 2024
Grant date	Jul 15, 2025
Priority date	—
Expiry date	Nov 8, 2044

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/178
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A construction method and an application of a miRNA electrochemical biosensor are provided. The construction method includes the following steps: fixing a SP substrate chain to a surface of AuNPs/GCE electrode, and performing sealing treatment with mercaptohexanol to obtain a miRNA electrochemical biosensor; a nucleotide sequence of the SP substrate chain is shown in SEQ ID NO:1, a 5′ end of the nucleotide sequence is modified with SH—(CH2)6—, and a ninth base of the nucleotide sequence is an rA cleavage site.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.