Method of preparing a microscope for imaging a sample

US12372770B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Manuel Amthor · Jena, DE
• Daniel Haase · Zöllnitz, DE
• Thomas Ohrt · Golmsdorf, DE

Key dates

• Filing date: Nov 3, 2020
• Grant date: Jul 29, 2025
• Expiry date: Nov 3, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/06
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method for preparing a microscope for imaging a sample. The method includes the following steps: providing the microscope for imaging the sample, wherein the microscope comprises an objective having a motor-adjustable objective correction ring for correcting imaging aberrations; acquiring sample information comprising at least one of the following indications: thickness of a cover glass, material of the cover glass, sample temperature, sample type, sample location on a sample carrier, embedding medium of the sample, or immersion medium; imaging the sample using the microscope for generating at least one raw image of the sample with a first setting of the objective correction ring; and inputting the at least one raw image and the sample information into a machine algorithm and determining a second setting of the objective correction ring, which reduces the imaging aberrations vis à vis the first setting, by means of the algorithm on the basis of the raw image and the sample information.