High-resolution spatial macromolecule abundance assessment

US12385033B2 · kind B2 · utility

Assignees

• The General Hospital Corporation · US
• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US
• President and Fellows of Harvard College · US

Inventors

• Evan Macosko · Cambridge, US
• Fei Chen · Thạnh Trung, VN
• Sam Rodriques · Cambridge, US
• Robert Stickels · Cambridge, US
• Caroline Anne Martin · Cambridge, US

Key dates

• Filing date: May 1, 2019
• Grant date: Aug 12, 2025
• Expiry date: Oct 6, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6841
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods for assessing relative macromolecule abundance (for example, RNA expression levels) in a spatially-defined manner across a tissue sample (for example, from brain, lung, liver, kidney, pancreas, and/or heart) are disclosed, specifically providing deep transcriptomic coverage at high-resolution (for example, at approximate 10 μm (single cell) resolution) across multiple locations assessed across the tissue sample.