Systems and methods for predicting repair outcomes in genetic engineering

US12406749B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US
• The Brigham and Women's Hospital, Inc. · US
• President and Fellows of Harvard College · US

Inventors

• Max Walt Shen · Cambridge, US
• Jonathan Hsu · Cambridge, US
• Mandana Arbab · Cambridge, US
• David K. Gifford · Cambridge, US
• David R. Liu · Lexington, US
• Richard Irving Sherwood · Cambridge, US

Key dates

• Filing date: Dec 15, 2018
• Grant date: Sep 2, 2025
• Expiry date: Dec 1, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The specification provides methods for introducing a desired genetic change in a nucleotide sequence using a double-strand break (DSB)-inducing genome editing system, the method comprising: identifying one or more available cut sites in a nucleotide sequence; analyzing the nucleotide sequence and available cut sites with a computational model to identify the optimal cut site for introducing the desired genetic change into the nucleotide sequence; and contacting the nucleotide sequence with a DSB-inducing genome editing system, thereby introducing the desired genetic change in the nucleotide sequence at the cut site.