Patent · US Active

Immunoassay-based determination of in-solution binding kinetics

US12416631B2 · kind B2 · utility

0Cited by

3References

6Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Uwe Dahl · Penzberg, DE
Gregor Jordan · Penzberg, DE
Roland Staack · Penzberg, DE

Key dates

Filing date	Nov 29, 2023
Grant date	Sep 16, 2025
Priority date	—
Expiry date	Nov 29, 2043

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/15
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Herein is reported a method for the determination of the binding affinity of a binder and its ligand comprising the step of determining based on the result of an immunoassay the fraction of free binder in a sample comprising binder, ligand and binder-ligand-complexes for at least two different binder:ligand ratios in the sample, and if the determined fraction of free binder is not comparable for all used binder:ligand ratios then the binder:ligand ratio in the sample is lowered and the sample is re-analyzed by the same immunoassay, and calculating based on the fraction of free binder in the previous step the binding affinity for the binder to its ligand.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.