Method for preparation of low-phenylalanine plastein
US4016147A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 16, 1976 |
| Grant date | Apr 5, 1977 |
| Priority date | — |
| Expiry date | Jan 16, 1996 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/857
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
Method for the preparation of a low-phenylalanine plastein comprising hydrolyzing stepwise a certain albumin or globulin protein with an endopeptidase having an affinity for the aromatic acid components, and an exopeptidase, subjecting the hydrolyzate thus-obtained to gel-filtration to divide it into two fractions, one being constituted with aromatic amino acids and the other being constituted with oligopeptides almost free from the aromatic amino acids, and then subjecting the latter fraction to usual plastein synthesis. Tyrosine and/or tryptophan may be added to the substrate at the plastein synthesis in order to incorporate them into the plastein molecules. The resultant plasteins may be purified through ultra-filtration in order to remove the remaining free amino acids and low-molecular peptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.