Determination of .beta.-lipoproteins in blood serum with polyanethole sulfonate
US4110077A · kind A · utility
19Cited by
12References
3Claims
0Family size
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Key dates
| Filing date | Oct 8, 1976 |
| Grant date | Aug 29, 1978 |
| Priority date | — |
| Expiry date | Oct 8, 1996 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/106664
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process of isolating beta-lipoproteins from blood serum in about 99% purity comprising precipitating beta-lipoproteins from blood serum substantially free of chylomicrons with polyanethole sulfonate and a divalent cation, forming a solution of the beta-lipoproteins and reprecipitating them with the divalent cation, again forming a solution of said beta-lipoproteins and passing them through a DEAE cellulose column. The beta-lipoproteins purified by the method are particularly suited as a calibration standard for use in assays of the beta-lipoprotein content of blood serum.
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