Process of separating from an aqueous medium a protein or a morphologically organized unit by liquid exclusion chromatography
US4199450A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Apr 26, 1978 |
| Grant date | Apr 22, 1980 |
| Priority date | — |
| Expiry date | Apr 26, 1998 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/826
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A novel process of separating from an aqueous medium at least one protein or at least one morphologically organized unit by liquid exclusion chromatography on a solid support previously passivated by treatment with an aqueous solution of a non-proteinic polymer. In this process, the chromatographic support is submitted, prior to the chromatographic operation, to a second passivation consisting in contacting the support with an aqueous solution of a protein which has a molecular weight lower than that of the protein to be separated, and which is adapted to be adsorbed by said support. The support and/or the liquid to be treated are sterilized with antiseptic agent selected from the group constituted by the lower aliphatic halogenated hydrocarbons.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.