Cultivatable human rotavirus type 2

US4341870A · kind A · utility

Assignee

• The United States of America as Represented by the Secretary of the Department of Health and Human Services · US

Inventors

• Richard G. Wyatt · Potomac, US
• Walter D. James · Beltsville, US
• Edward H. Bohl · Wooster, US
• Kenneth W. Theil · Wooster, US
• Linda J. Saif · West Salem, US
• Anthony R. Kalica · Rockville, US
• Harry Greenberg · Washington, US
• Albert Z. Kapikian · Rockville, US
• Robert M. Chanock · Bethesda, US

Key dates

• Filing date: Nov 19, 1980
• Grant date: Jul 27, 1982
• Expiry date: Nov 19, 2000

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2720/12364
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention relates to a precursor or intermediate for a rotavirus vaccine to protect infants and young children against diarrhea caused by rotavirus. The invention is the efficient propagation of a strain of human rotavirus type 2 prepared by a method of cultivation by multiple passages in vivo in gnotobiotic piglets and by multiple passages in vitro subsequently in AGMK (African green monkey kidney) cell cultures. Additionally, rotavirus is treated with a biologically active amount of trypsin prior to growth in AGMK cells, and the inoculated cell cultures are centrifuged at a low centrifugal force before incubation.