Recombinant DNA process utilizing a papilloma virus DNA as a vector
US4419446A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 31, 1980 |
| Grant date | Dec 6, 1983 |
| Priority date | — |
| Expiry date | Dec 31, 2000 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/20043
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel method and composition are provided for the replication and expression of exogenous genes in eukaryotic cells. A segment of a papilloma virus genome capable of extrachromosomal replication is linked to the foreign gene(s) using recombinant DNA techniques to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a eukaryotic cell by transformation, and the isolation of transformant provides cells for replication and expression of the DNA molecules present in the modified plasmid. The transforming region of the bovine papilloma virus provides a unique vector in that it provides both the capability of autonomous extrachromosomal replication but also the malignant transformed phenotype. Thus, genes which of themselves provide no selectable phenotypical property can be conveniently and efficiently introduced into eukaryotic cells and the transformants selected. The method is useful in that the foreign DNA is faithfully expressed and the gene products (proteins), such as pro-insulin (an insulin precursor) is synthesized.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.