Vitro cellular interaction with amnion membrane substrate

US4446234A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Raimondo Russo · Milano, IT
• Lance A. Liotta · Bethesda, US

Key dates

• Filing date: Oct 23, 1981
• Grant date: May 1, 1984
• Expiry date: Oct 23, 2001

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/92
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Amnion membranes derived from mammalian placentas are employed as the substrate tissue in in vitro assay methods for evaluating cellular interaction with natural barrier tissues. The amnion is also employed as a growth substrate, particularly for the culture of cells difficult to culture on conventional substrates. A diagnostic apparatus including an amnion membrane is also disclosed.