Isoelectric focussing-polynucleotide/polyacrylamide gel electrophoresis
US4495279A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 27, 1982 |
| Grant date | Jan 22, 1985 |
| Priority date | — |
| Expiry date | Jul 27, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/922
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An electrophoresis system which provides enhanced resolution and ability to identify nucleases through use of a two-dimensional technique involving isoelectric focussing of tube gels followed by the electrophoresis of second-dimension slab gels formed by the use of a holder allowing slab gels to be cast directly on the sides of the tube gels. DNA is employed as the substrate for casting the slab gels. The slab gels are electrophoresed for the second dimension in a chamber wherein the rack containing a stack of slab gels forms the partition between the anolyte and catholyte compartments. After the second-dimension electrophoresis, the slab gels are incubated in incubation buffer and placed in Pyronin Y to stain the unhydrolized DNA. After staining, they are destained in acetic acid. The DNAses are then visible as colorless spots in a reddish-colored gel.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.