Purification and activity assurance of precipitated heterologous proteins
US4518526A · kind A · utility
107Cited by
3References
11Claims
0Family size
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Key dates
| Filing date | Jun 1, 1984 |
| Grant date | May 21, 1985 |
| Priority date | — |
| Expiry date | Jun 1, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/826
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for isolating and purifying a precipitated heterologous protein from a host cell culture, including treating the host cell culture with a buffered solution of ionic strength suitable to solubilize most of the host protein but in which refractile heterologous protein is substantially insoluble, and disrupting the cells to form a supernatant and an insoluble fraction; treating the insoluble fraction with a strongly denaturing solution to solubilize the refractile heterologous protein; and recovering renatured heterologous protein.
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