Process for obtaining lipid envelope virus sub-units, notably antigens for use as vaccines, the products obtained and their applications
US4522809A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 1, 1984 |
| Grant date | Jun 11, 1985 |
| Priority date | — |
| Expiry date | Aug 1, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/16234
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process is disclosed for producing virus sub-units with lipid envelopes, comprising, in a neutral or basic pH, dissolving a lower halogenated hydrocarbon, at a concentration equivalent to or approximating its limit of solubility, in an aqueous virus suspension, thereafter contacting, with stirring, the preparation so obtained with a nonionic detergent at a minimum concentration effective to induce disruption of virions into heavy sub-units for a length of time sufficient to permit said disruption, and in separating the heavy sub-units so obtained from the reaction medium. The process permits preparation of purified disrupted antigens for use as vaccines, notably influenza vaccines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.